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in fusion cloning technology  (TaKaRa)


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    Structured Review

    TaKaRa in fusion cloning technology
    In Fusion Cloning Technology, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1543 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/in+fusion+technology/In-Fusion+HD+Cloning+Plus/pm42133578-194-18-21
    Average 99 stars, based on 1543 article reviews
    in fusion cloning technology - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cloning:

    Article Title: Identification of a cytosol to nucleus feedback loop that regulates neuronal microtubule nucleation
    Article Snippet: Class I EB1 videos were collected on Zeiss Widefield Axio Imager.M2 with an AxioCam 506 mono camera and Plan-APOCHROMAT 63x oil DIC (1.4 NA) objective. .. Cloning strategies involved either In-Fusion technology (Takara In-Fusion Kit 638948) or ligation-mediated cloning using T4 ligase (NEB M0202S). .. Fill-in reactions for blunting were carried out with Large Klenow fragment (NEB M0210S).

    Ligation:

    Article Title: Identification of a cytosol to nucleus feedback loop that regulates neuronal microtubule nucleation
    Article Snippet: Class I EB1 videos were collected on Zeiss Widefield Axio Imager.M2 with an AxioCam 506 mono camera and Plan-APOCHROMAT 63x oil DIC (1.4 NA) objective. .. Cloning strategies involved either In-Fusion technology (Takara In-Fusion Kit 638948) or ligation-mediated cloning using T4 ligase (NEB M0202S). .. Fill-in reactions for blunting were carried out with Large Klenow fragment (NEB M0210S).

    Construct:

    Article Title: Evolutionary-conserved RLF, a cytochrome b 5 -like heme-binding protein, regulates organ development in Marchantia polymorpha.
    Article Snippet: .. To generate RLFpro:RLF-GFP, RLFpro: RLF(H161G)-GFP, RLFpro:RLF(H184G)-GFP, and RLFpro:RLF (H161G/H184G)-GFP constructs, the promoter region of RLF (RLFpro) (804 bp) was amplified from A. thaliana WT genomic DNA using IF-pGWB501RLFpro-F and IF-pGWB501RLFproR primers and cloned into pGWB501 using the In-Fusion technology (pGWB501 RLFpro; Takara Bio Inc., Shiga, Japan). .. Using the previously synthesized coding sequence (CDS) of full-length RLF (RLFcds) (Ikeyama et al., 2010) as a template, the CACC-RLFcds-XhoI fragment was amplified using CACC-RLF-F and RLF-XhoI-R primers and cloned into the pENTR/D-TOPO cloning vector (Thermo Fisher Scientific, Rockville, MD, USA) to produce the pENTR-RLF-XhoI vector.

    Article Title: Evolutionary‐conserved RLF , a cytochrome b 5 ‐like heme‐binding protein, regulates organ development in Marchantia polymorpha
    Article Snippet: .. To generate RLFpro:RLF‐GFP, RLFpro:RLF(H161G)‐GFP , RLFpro:RLF(H184G)‐GFP , and RLFpro:RLF(H161G/H184G)‐GFP constructs, the promoter region of RLF ( RLFpro ) (804 bp) was amplified from A. thaliana WT genomic DNA using IF‐pGWB501RLFpro‐F and IF‐pGWB501RLFpro‐R primers and cloned into pGWB501 using the In‐Fusion technology (pGWB501 RLFpro ; Takara Bio Inc., Shiga, Japan). .. Using the previously synthesized coding sequence (CDS) of full‐length RLF ( RLFcds ) (Ikeyama et al ., ) as a template, the CACC‐RLFcds‐XhoI fragment was amplified using CACC‐RLF‐F and RLF‐XhoI‐R primers and cloned into the pENTR/D‐TOPO cloning vector (Thermo Fisher Scientific, Rockville, MD, USA) to produce the pENTR‐ RLF‐XhoI vector.

    Article Title: Evolutionary conserved RLF, a plant cytochrome b 5 -like heme-binding protein, is essential for organ development in Marchantia polymorpha
    Article Snippet: .. To generate RLFpro:RLF-GFP, RLFpro:RLF(H161G)-GFP , RLFpro:RLF(H184G)-GFP , and RLFpro:RLF(H161G/H184G)-GFP constructs, the promoter region of RLF ( RLFpro ) (804 bp) was amplified from A. thaliana wild-type genomic DNA using IF-pGWB501RLFpro-F and IF-pGWB501RLFpro-R primers, and cloned into pGWB501 using In-Fusion technology (Takara Bio Inc., Japan; pGWB501 RLFpro ). .. Using previously synthesized coding sequence of full-length RLF ( RLFcds ) ( Ikeyama et al ., 2010 ) as a template, the CACC-RLFcds-XhoI fragment was amplified using CACC-RLF-F and RLF-XhoI-R primers, and cloned into the pENTR/D-TOPO cloning vector (Life Technologies, Rockville, MD, USA) to produce the pENTR- RLF-XhoI vector.

    Amplification:

    Article Title: Evolutionary-conserved RLF, a cytochrome b 5 -like heme-binding protein, regulates organ development in Marchantia polymorpha.
    Article Snippet: .. To generate RLFpro:RLF-GFP, RLFpro: RLF(H161G)-GFP, RLFpro:RLF(H184G)-GFP, and RLFpro:RLF (H161G/H184G)-GFP constructs, the promoter region of RLF (RLFpro) (804 bp) was amplified from A. thaliana WT genomic DNA using IF-pGWB501RLFpro-F and IF-pGWB501RLFproR primers and cloned into pGWB501 using the In-Fusion technology (pGWB501 RLFpro; Takara Bio Inc., Shiga, Japan). .. Using the previously synthesized coding sequence (CDS) of full-length RLF (RLFcds) (Ikeyama et al., 2010) as a template, the CACC-RLFcds-XhoI fragment was amplified using CACC-RLF-F and RLF-XhoI-R primers and cloned into the pENTR/D-TOPO cloning vector (Thermo Fisher Scientific, Rockville, MD, USA) to produce the pENTR-RLF-XhoI vector.

    Article Title: Evolutionary‐conserved RLF , a cytochrome b 5 ‐like heme‐binding protein, regulates organ development in Marchantia polymorpha
    Article Snippet: .. To generate RLFpro:RLF‐GFP, RLFpro:RLF(H161G)‐GFP , RLFpro:RLF(H184G)‐GFP , and RLFpro:RLF(H161G/H184G)‐GFP constructs, the promoter region of RLF ( RLFpro ) (804 bp) was amplified from A. thaliana WT genomic DNA using IF‐pGWB501RLFpro‐F and IF‐pGWB501RLFpro‐R primers and cloned into pGWB501 using the In‐Fusion technology (pGWB501 RLFpro ; Takara Bio Inc., Shiga, Japan). .. Using the previously synthesized coding sequence (CDS) of full‐length RLF ( RLFcds ) (Ikeyama et al ., ) as a template, the CACC‐RLFcds‐XhoI fragment was amplified using CACC‐RLF‐F and RLF‐XhoI‐R primers and cloned into the pENTR/D‐TOPO cloning vector (Thermo Fisher Scientific, Rockville, MD, USA) to produce the pENTR‐ RLF‐XhoI vector.

    Article Title: Evolutionary conserved RLF, a plant cytochrome b 5 -like heme-binding protein, is essential for organ development in Marchantia polymorpha
    Article Snippet: .. To generate RLFpro:RLF-GFP, RLFpro:RLF(H161G)-GFP , RLFpro:RLF(H184G)-GFP , and RLFpro:RLF(H161G/H184G)-GFP constructs, the promoter region of RLF ( RLFpro ) (804 bp) was amplified from A. thaliana wild-type genomic DNA using IF-pGWB501RLFpro-F and IF-pGWB501RLFpro-R primers, and cloned into pGWB501 using In-Fusion technology (Takara Bio Inc., Japan; pGWB501 RLFpro ). .. Using previously synthesized coding sequence of full-length RLF ( RLFcds ) ( Ikeyama et al ., 2010 ) as a template, the CACC-RLFcds-XhoI fragment was amplified using CACC-RLF-F and RLF-XhoI-R primers, and cloned into the pENTR/D-TOPO cloning vector (Life Technologies, Rockville, MD, USA) to produce the pENTR- RLF-XhoI vector.

    Clone Assay:

    Article Title: Evolutionary-conserved RLF, a cytochrome b 5 -like heme-binding protein, regulates organ development in Marchantia polymorpha.
    Article Snippet: .. To generate RLFpro:RLF-GFP, RLFpro: RLF(H161G)-GFP, RLFpro:RLF(H184G)-GFP, and RLFpro:RLF (H161G/H184G)-GFP constructs, the promoter region of RLF (RLFpro) (804 bp) was amplified from A. thaliana WT genomic DNA using IF-pGWB501RLFpro-F and IF-pGWB501RLFproR primers and cloned into pGWB501 using the In-Fusion technology (pGWB501 RLFpro; Takara Bio Inc., Shiga, Japan). .. Using the previously synthesized coding sequence (CDS) of full-length RLF (RLFcds) (Ikeyama et al., 2010) as a template, the CACC-RLFcds-XhoI fragment was amplified using CACC-RLF-F and RLF-XhoI-R primers and cloned into the pENTR/D-TOPO cloning vector (Thermo Fisher Scientific, Rockville, MD, USA) to produce the pENTR-RLF-XhoI vector.

    Article Title: Evolutionary‐conserved RLF , a cytochrome b 5 ‐like heme‐binding protein, regulates organ development in Marchantia polymorpha
    Article Snippet: .. To generate RLFpro:RLF‐GFP, RLFpro:RLF(H161G)‐GFP , RLFpro:RLF(H184G)‐GFP , and RLFpro:RLF(H161G/H184G)‐GFP constructs, the promoter region of RLF ( RLFpro ) (804 bp) was amplified from A. thaliana WT genomic DNA using IF‐pGWB501RLFpro‐F and IF‐pGWB501RLFpro‐R primers and cloned into pGWB501 using the In‐Fusion technology (pGWB501 RLFpro ; Takara Bio Inc., Shiga, Japan). .. Using the previously synthesized coding sequence (CDS) of full‐length RLF ( RLFcds ) (Ikeyama et al ., ) as a template, the CACC‐RLFcds‐XhoI fragment was amplified using CACC‐RLF‐F and RLF‐XhoI‐R primers and cloned into the pENTR/D‐TOPO cloning vector (Thermo Fisher Scientific, Rockville, MD, USA) to produce the pENTR‐ RLF‐XhoI vector.

    Article Title: Evolutionary conserved RLF, a plant cytochrome b 5 -like heme-binding protein, is essential for organ development in Marchantia polymorpha
    Article Snippet: .. To generate RLFpro:RLF-GFP, RLFpro:RLF(H161G)-GFP , RLFpro:RLF(H184G)-GFP , and RLFpro:RLF(H161G/H184G)-GFP constructs, the promoter region of RLF ( RLFpro ) (804 bp) was amplified from A. thaliana wild-type genomic DNA using IF-pGWB501RLFpro-F and IF-pGWB501RLFpro-R primers, and cloned into pGWB501 using In-Fusion technology (Takara Bio Inc., Japan; pGWB501 RLFpro ). .. Using previously synthesized coding sequence of full-length RLF ( RLFcds ) ( Ikeyama et al ., 2010 ) as a template, the CACC-RLFcds-XhoI fragment was amplified using CACC-RLF-F and RLF-XhoI-R primers, and cloned into the pENTR/D-TOPO cloning vector (Life Technologies, Rockville, MD, USA) to produce the pENTR- RLF-XhoI vector.

    other:

    Article Title: USP7 deubiquitinase stabilizes FAN1 to support DNA crosslink repair and suppress CAG repeat expansion
    Article Snippet: Cells were transfected in tandem with both plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol.



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